Seyi Makinde was born at Catholic Hospital Oluyoro, Ibadan, Oyo State to late Pa Olatubosun Makinde, a teacher who later trained as an accountant, and Madam Abigail Makinde, a telephone operator in the civil service, of Aigbofa Compound, Oja’ba Ibadan, Oyo State.
He attended St. Michael’s Primary School, Yemetu in Ibadan and Bishop Phillips Academy, Iwo Road Ibadan. He also attended the University of Lagos, Akoka Yaba, Lagos and graduated with a B.Sc. (Hons) degree in Electrical Engineering in 1990. He was trained in Industrial Control Services in Houston, Texas in 1998; and Development of Analytical Competence at Lagos Business School (now Pan African University) in 1999. He studied Fundamentals of Crude Sampling at Jiskoot Auto Control Training Centre, Kent England in 2002, and Understanding and Solving Complex Business Problems at Massachusetts Institute of Technology, USA in 2005.